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human breast cancer mirna microarray  (Qiagen)


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    Qiagen human breast cancer mirna microarray
    Effects of melatonin and radiation on breast cancer <t>miRNA</t> expression <t>microarray.</t> Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray <t>(MIHS-109ZA).</t> ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.
    Human Breast Cancer Mirna Microarray, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human breast cancer mirna microarray/product/Qiagen
    Average 90 stars, based on 1 article reviews
    human breast cancer mirna microarray - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "Melatonin Modulation of Radiation-Induced Molecular Changes in MCF-7 Human Breast Cancer Cells"

    Article Title: Melatonin Modulation of Radiation-Induced Molecular Changes in MCF-7 Human Breast Cancer Cells

    Journal: Biomedicines

    doi: 10.3390/biomedicines10051088

    Effects of melatonin and radiation on breast cancer miRNA expression microarray. Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.
    Figure Legend Snippet: Effects of melatonin and radiation on breast cancer miRNA expression microarray. Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.

    Techniques Used: Expressing, Microarray, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Control

    Changes in miRNA expression levels in MCF-7 cells. Cells were pre-treated with 1 nM melatonin for 1 week prior to being irradiated (8 Gy), and after 4 h total RNA was isolated and reverse transcribed; ( a ) RT-PCR analysis of selected miRNAs (miR-20a, miR-19a, miR-93, miR-20b, miR-29a, miR10a and miR10b) where the pre-treatment with melatonin potentiated the inhibitory effect of radiation; ( b ) RT-PCR analysis of selected miRNAs (miR-17, miR-141 and miR-15a) induced by radiation and counteracted by melatonin pre-treatment; ( c ) RT-PCR analysis of miR34a stimulated by radiation and melatonin. Data are expressed as relative normalized expression compared to control cells (mean ± S.E.M.). a, p ≤ 0.001 vs. C; b, p ≤ 0.001 vs. R; c, p ≤ 0.05 vs. R; d, p ≤ 0.01 vs. R.
    Figure Legend Snippet: Changes in miRNA expression levels in MCF-7 cells. Cells were pre-treated with 1 nM melatonin for 1 week prior to being irradiated (8 Gy), and after 4 h total RNA was isolated and reverse transcribed; ( a ) RT-PCR analysis of selected miRNAs (miR-20a, miR-19a, miR-93, miR-20b, miR-29a, miR10a and miR10b) where the pre-treatment with melatonin potentiated the inhibitory effect of radiation; ( b ) RT-PCR analysis of selected miRNAs (miR-17, miR-141 and miR-15a) induced by radiation and counteracted by melatonin pre-treatment; ( c ) RT-PCR analysis of miR34a stimulated by radiation and melatonin. Data are expressed as relative normalized expression compared to control cells (mean ± S.E.M.). a, p ≤ 0.001 vs. C; b, p ≤ 0.001 vs. R; c, p ≤ 0.05 vs. R; d, p ≤ 0.01 vs. R.

    Techniques Used: Expressing, Irradiation, Isolation, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Control



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    human breast cancer mirna microarray  (Qiagen)
    90
    Qiagen human breast cancer mirna microarray
    Effects of melatonin and radiation on breast cancer <t>miRNA</t> expression <t>microarray.</t> Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray <t>(MIHS-109ZA).</t> ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.
    Human Breast Cancer Mirna Microarray, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human breast cancer mirna microarray/product/Qiagen
    Average 90 stars, based on 1 article reviews
    human breast cancer mirna microarray - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Effects of melatonin and radiation on breast cancer miRNA expression microarray. Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.

    Journal: Biomedicines

    Article Title: Melatonin Modulation of Radiation-Induced Molecular Changes in MCF-7 Human Breast Cancer Cells

    doi: 10.3390/biomedicines10051088

    Figure Lengend Snippet: Effects of melatonin and radiation on breast cancer miRNA expression microarray. Total RNA from MCF-7 cells was extracted 4 h after radiation, reverse transcribed and used for RT-PCR analysis using Human Breast Cancer microarray (MIHS-109ZA). ( a – c ) Heatmaps of relative normalized expression between different treatments ( a ) M vs. C; ( b ) R vs. C; ( c ) M + R vs. C; ( d ) Bar chart of relative normalized expression (ΔCt) of selected miRNAs. a: miR20a; b: miR-20b; c: miR-17; d: miR-141; e: miR-15a; f: miR-19a; g: miR29a; h: miR-93; i: miR-10b. C: Control; M: Melatonin pre-treated cells (1 nM); R: Radiated cells (8 Gy); M + R: Melatonin pre-treated and radiated cells.

    Article Snippet: We next analyzed the influence of melatonin on the expression of multiple genes and miRNAs involved in breast cancer using a gene microarray (Human Breast Cancer RT 2 Profiler TM PCR Array) and a Human Breast Cancer miRNA microarray (MIHS-109ZA, Qiagen, Germantown, MD, USA).

    Techniques: Expressing, Microarray, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Control

    Changes in miRNA expression levels in MCF-7 cells. Cells were pre-treated with 1 nM melatonin for 1 week prior to being irradiated (8 Gy), and after 4 h total RNA was isolated and reverse transcribed; ( a ) RT-PCR analysis of selected miRNAs (miR-20a, miR-19a, miR-93, miR-20b, miR-29a, miR10a and miR10b) where the pre-treatment with melatonin potentiated the inhibitory effect of radiation; ( b ) RT-PCR analysis of selected miRNAs (miR-17, miR-141 and miR-15a) induced by radiation and counteracted by melatonin pre-treatment; ( c ) RT-PCR analysis of miR34a stimulated by radiation and melatonin. Data are expressed as relative normalized expression compared to control cells (mean ± S.E.M.). a, p ≤ 0.001 vs. C; b, p ≤ 0.001 vs. R; c, p ≤ 0.05 vs. R; d, p ≤ 0.01 vs. R.

    Journal: Biomedicines

    Article Title: Melatonin Modulation of Radiation-Induced Molecular Changes in MCF-7 Human Breast Cancer Cells

    doi: 10.3390/biomedicines10051088

    Figure Lengend Snippet: Changes in miRNA expression levels in MCF-7 cells. Cells were pre-treated with 1 nM melatonin for 1 week prior to being irradiated (8 Gy), and after 4 h total RNA was isolated and reverse transcribed; ( a ) RT-PCR analysis of selected miRNAs (miR-20a, miR-19a, miR-93, miR-20b, miR-29a, miR10a and miR10b) where the pre-treatment with melatonin potentiated the inhibitory effect of radiation; ( b ) RT-PCR analysis of selected miRNAs (miR-17, miR-141 and miR-15a) induced by radiation and counteracted by melatonin pre-treatment; ( c ) RT-PCR analysis of miR34a stimulated by radiation and melatonin. Data are expressed as relative normalized expression compared to control cells (mean ± S.E.M.). a, p ≤ 0.001 vs. C; b, p ≤ 0.001 vs. R; c, p ≤ 0.05 vs. R; d, p ≤ 0.01 vs. R.

    Article Snippet: We next analyzed the influence of melatonin on the expression of multiple genes and miRNAs involved in breast cancer using a gene microarray (Human Breast Cancer RT 2 Profiler TM PCR Array) and a Human Breast Cancer miRNA microarray (MIHS-109ZA, Qiagen, Germantown, MD, USA).

    Techniques: Expressing, Irradiation, Isolation, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Control